Recombinant Anti-CD8A (aa 22-182) Antibody (V3S-1022-YC2461) (CAT#: V3S-1022-YC2461)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant Mouse antibody provided by CreativeBiolabs. The antibody is specific for CD8a molecule. It can be used for CD8a molecule detection in Flow Cytometry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Immunogen Recombinant extracellular domain of human CD8a protein (Ser 22 - Asp 182) fused with a C-terminal polyhistidine tag
Epitope In the amino acids 22 to 182.
Isotype IgG

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application FC

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target CD8A
Alternative Name CD8a Molecule; T-Lymphocyte Differentiation Antigen T8/Leu-2; CD8 Antigen, Alpha Polypeptide (P32); MAL; T-Cell Surface Glycoprotein CD8 Alpha Chain; Leu2 T-Lymphocyte Antigen; OKT8 T-Cell Antigen; T-Cell Antigen Leu2; T Cell Co-Receptor; T8 T-Cell Antigen; CD8a Antigen; Leu2; P32; CD8
Gene ID 925
UniProt P01732
Research Area Immunology; Stem Cell
Related Disease Tumor
For research use only, not directly for clinical use.
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