| Description | This product is a recombinant Mouse antibody provided by CreativeBiolabs. The antibody is specific for CD8a molecule. It can be used for CD8a molecule detection in Flow Cytometry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Recombinant extracellular domain of human CD8a protein (Ser 22 - Asp 182) fused with a C-terminal polyhistidine tag |
| Epitope | In the amino acids 22 to 182. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | FC |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CD8A |
| Alternative Name | CD8a Molecule; T-Lymphocyte Differentiation Antigen T8/Leu-2; CD8 Antigen, Alpha Polypeptide (P32); MAL; T-Cell Surface Glycoprotein CD8 Alpha Chain; Leu2 T-Lymphocyte Antigen; OKT8 T-Cell Antigen; T-Cell Antigen Leu2; T Cell Co-Receptor; T8 T-Cell Antigen; CD8a Antigen; Leu2; P32; CD8 |
| Gene ID | 925 |
| UniProt | P01732 |
| Research Area | Immunology; Stem Cell |
| Related Disease | Tumor |
