| Description | This product is a monoclonal antibody derived from mouse, which can specifically recognize FGFR2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | A 1: 1 mixture of human FGFR2 IgD2-IgD3 (Illb) and human FGFR2 IgD2-IgD3 (IIIc) fused with a human Fc moiety at their C-termini |
| Epitope | In the IgD2-D3 region |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; FC |
| Application Notes | The antibody is recommended for detection of FGFR2 by ELISA, FC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | FGFR2 |
| Alternative Name | FGFR2; fibroblast growth factor receptor 2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM; FGFR-2; FGF receptor; soluble FGFR4 variant 4; bacteria-expressed kinase; hydroxyaryl-protein kinase; FGFR2-AHCYL1 fusion kinase protein; keratinocyte growth factor receptor; BEK fibroblast growth factor receptor; protein tyrosine kinase, receptor like 14 |
| Gene ID | 2263 |
| UniProt | P21802 |
| Research Area | Cardiovascular |
Figure 1 Recombinant Anti-Human FGFR2 Antibody (V3S-0622-YC1623) in WB
Western blot analysis of V3S-0622-YC1623 was performed by loading recombinant human FGFR2 protein.
V3S-0622-YC1623 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-mouse IgG
Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)
Figure 1 Recombinant Anti-Human FGFR2 Antibody (V3S-0622-YC1623) in WB
Figure 2 Recombinant Anti-Human FGFR2 Antibody (V3S-0622-YC1623) in ELISA
ELISA analysis of V3S-0622-YC1623 was performed by coating with recombinant human FGFR2 protein. Then blocked with BSA and incubated with Anti-Human FGFR2 Recombinant Antibody (GP369/4B9) (V3S-0622-YC1623). The Goat Anti-mouse IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Recombinant Anti-Human FGFR2 Antibody (V3S-0622-YC1623) in ELISA
