| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize TNF receptor superfamily member 8. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | pHRm30c and sCD30 protein |
| Epitope | The epitope is in the membrane- specific Ep2 (amino acids 107-153). |
| Affinity | KD = 1.1 nM, determined by surface plasmon resonance. |
| Isotype | IgG1, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | DB; ELISA; FC; WB |
| Application Notes | The antibody is recommended for detection of TNFRSF8 by ELISA, FC, WB assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Mouse Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1961) in ELISA
ELISA analysis of V3S-0522-YC1961 was performed by coating with Recombinant Human TNFRSF8.
The secondary antibody: HRP-anti-mouse IgG (H+L)
Figure 1 Mouse Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1961) in ELISA
Figure 2 Mouse Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1961) in WB
Western blot analysis of V3S-0522-YC1961 was performed by loading Recombinant Human TNFRSF8 (His Tag).
The incubation concentration of V3S-0522-YC1961 is 2 ng/μL.
The secondary antibody: HRP-anti-mouse IgG (H+L)
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 2 Mouse Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1961) in WB
Figure 3 Mouse Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1961) in DB
Dot blot analysis of V3S-0522-YC1961 was performed by loading Recombinant Human TNFRSF8 (His Tag).
The incubation concentration of V3S-0522-YC1961 is 2 μg/mL.
The secondary antibody: HRP-anti-mouse IgG (H+L)
Figure 3 Mouse Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1961) in DB
