| Description | This product is a monoclonal antibody derived from mouse, which can specifically recognize SNCA. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | α-synuclein fibrils |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; DB; FuncS |
| Application Notes | The antibody is recommended for detection of SNCA by FuncS assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-SNCA Monoclonal Antibody (V3S-0622-YC2573) in SDS-PAGE
SDS-PAGE analysis of V3S-0622-YC2573 in β-mercaptoethanol-reduced (Lane 1, 3.5 μg) and non-reduced (Lane 2, 2 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of proteins (heavy chain and light chain) in different condition migrate as about 50 kDa and 25 kDa, respectively. Non-reduced protein migrates about 200 kDa.
Figure 1 Anti-SNCA Monoclonal Antibody (V3S-0622-YC2573) in SDS-PAGE
Figure 2 Anti-SNCA Monoclonal Antibody (V3S-0622-YC2573) in SEC-HPLC
The purity of V3S-0622-YC2573 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 2 Anti-SNCA Monoclonal Antibody (V3S-0622-YC2573) in SEC-HPLC
Figure 3 Anti-SNCA Monoclonal Antibody (V3S-0622-YC2573) in WB
Western blot analysis of V3S-0622-YC2573 was performed by loading Recombinant human SNCA protein in non-reduced (Lane 1, 2 μg) and reduced (Lane 2, 2 μg) condition. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC2573 (2 μg/ml) and HRP-conjugated goat anti-mouse IgG as a secondary antibody (1:5000). Chemiluminescent detection was performed.
Figure 3 Anti-SNCA Monoclonal Antibody (V3S-0622-YC2573) in WB
Figure 4 Anti-SNCA Monoclonal Antibody (V3S-0622-YC2573) in ELISA
ELISA analysis of V3S-0622-YC2573 was performed by coating with Recombinant human SNCA protein. Then blocked with BSA, and incubated with V3S-0622-YC2573. The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1:5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-SNCA Monoclonal Antibody (V3S-0622-YC2573) in ELISA
Figure 5 Anti-SNCA Monoclonal Antibody (V3S-0622-YC2573) in DB
Dot blot analysis of V3S-0622-YC2573 was performed by coating with Recombinant human SNCA protein and incubated with V3S-0622-YC2573 (2 μg/ml). The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1:5000).
Figure 5 Anti-SNCA Monoclonal Antibody (V3S-0622-YC2573) in DB
