Anti-SNCA Neutralizing Antibody (V3S-0622-YC2573) (CAT#: V3S-0622-YC2573)

Figure 1 Anti-SNCA Monoclonal Antibody (V3S-0622-YC2573) in SDS-PAGE

SDS-PAGE analysis of V3S-0622-YC2573 in β-mercaptoethanol-reduced (Lane 1, 3.5 μg) and non-reduced (Lane 2, 2 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of proteins (heavy chain and light chain) in different condition migrate as about 50 kDa and 25 kDa, respectively. Non-reduced protein migrates about 200 kDa.

Figure 2 Anti-SNCA Monoclonal Antibody (V3S-0622-YC2573) in SEC-HPLC

The purity of V3S-0622-YC2573 was greater than 95% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Anti-SNCA Monoclonal Antibody (V3S-0622-YC2573) in WB

Western blot analysis of V3S-0622-YC2573 was performed by loading Recombinant human SNCA protein in non-reduced (Lane 1, 2 μg) and reduced (Lane 2, 2 μg) condition. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC2573 (2 μg/ml) and HRP-conjugated goat anti-mouse IgG as a secondary antibody (1:5000). Chemiluminescent detection was performed.

Figure 4 Anti-SNCA Monoclonal Antibody (V3S-0622-YC2573) in ELISA

ELISA analysis of V3S-0622-YC2573 was performed by coating with Recombinant human SNCA protein. Then blocked with BSA, and incubated with V3S-0622-YC2573. The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1:5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 5 Anti-SNCA Monoclonal Antibody (V3S-0622-YC2573) in DB

Dot blot analysis of V3S-0622-YC2573 was performed by coating with Recombinant human SNCA protein and incubated with V3S-0622-YC2573 (2 μg/ml). The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1:5000).