| Description | This product is a receptor-blocking human monoclonal antibody. It retained potent in vitro neutralizing activity against SARS-CoV-2 variants including the Alpha, Beta, Gamma, Delta and Omicron VOCs. The antibody exhibits potent neutralizing efficacy against Wuhan-Hu-1 S mediated cell entry. The mAb could also protect mice and hamsters prophylactically against challenge with all current SARS-CoV-2 VOCs, and shows therapeutic activity against SARS-CoV-2 challenge in both animal models. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 (Alpha, Beta, Gamma, Delta, Omicron BA.1 and BA.2) |
| Immunogen | SARS-CoV-2 spike protein |
| Epitope | An epitope containing residues Y421, L455, F456, F486 and Y489 of RBD domain. |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; BLI; Neut |
| Application Notes | The antibody was found to block the binding of recombinant S trimer to ACE2 by BLI and ELISA-based assay. ELISA: The antibody started at 10 μg/mL with a serial 1: 5 dilution. Bio-layer interferometry (BLI): The biosensor tips were immersed into a well containing mAb (50 μg/mL) for 15 min. Live virus neutralization: the mAb potently neutralized D614G (IC50: 5.7 ng/ml), Wuhan-Hu-1 (IC50: 5.4 ng/ml), Lambda and Mu variants (IC50s: 1.2 and 4.8 ng/ml, respectively),as well as Alpha, Beta, Gamma, Delta and Omicron (BA.1/BA.2) with IC₅₀ values ranging from 3.1 to 12.5 ng/ml. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SARS-CoV-2 S1 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 Spike S1 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
