| Description | This product is a human neutralizing antibody clone CH55 that targets HIV-1 gp120. It is specific for CD4-induced envelope (Env) epitopes within constant region 1 and 2 (C1/C2). CH55 exhibits binding affinity and neutralization potency against HIV-1. It has been detected by Enzyme-linked immunosorbent assay, Flow cytometry and Neutralization assay. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | HIV-1 |
| Epitope | HIV-1 gp120 C1/C2 Epitope |
| Affinity | 1.9 nM |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; FC; Neut |
| Application Notes | Flow Cytometry (FC): Serially diluted antibody ranging from 10 to 0.0001 μg/ml was then incubated with the cells for 20 min at room temperature before being washed twice. Neutralization assay (Neut): Serially diluted antibody (ranging from 10 to 0.0003 μg/mL) was incubated with target and effector cells at a ratio of 50:1 effectors to targets. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HIV-1 gp120 |
| Alternative Name | Human immunodeficiencyvirus-1 glycoprotein 120 |
| Research Area | Infectious Disease |
| Related Disease | Acquired Immune Deficiency Syndrome |
