| Description | This is a bovine neutralizing antibody clone NC-Cow8 specific for HIV-1 gp120 (Human immunodeficiencyvirus-1 glycoprotein 120). NC-Cow8 has binding affinity with HIV-1 gp120 and it is a broadly neutralizing antibody. |
| Clonality | Monoclonal |
| Host Species | Bovine |
| Target Species | HIV-1 |
| Immunogen | Soluble cleaved HIV envelope trimer protein BG505 SOSIP |
| Epitope | CD4bs |
| Isotype | Bovine IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Neut; FC |
| Application Notes | ELISA: Serial dilutions of mAb was added to the wells and plates were incubated at room temperature for 1 hour. Neutralization assay (Neut): Neutralizing activity was measured by incubating monoclonal antibody or sera with replication incompetent pseudovirus for 1 h at 37°C. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HIV-1 gp120 |
| Alternative Name | Human immunodeficiencyvirus-1 glycoprotein 120 |
| Research Area | Infectious Disease |
| Related Disease | Acquired Immune Deficiency Syndrome |
