| Description | This product is a monkey nuetralizing antibody clone J038, which binding Human immunodeficiencyvirus-1 glycoprotein 120 (HIV-1 gp120). J038 binds to the V2-Apex of gp120. It has potent and broad neutralizing efficiency. It can neutralizes 54% of global circulating HIV-1 strains. |
| Clonality | Monoclonal |
| Host Species | Monkey |
| Target Species | HIV-1 |
| Immunogen | SHIV1157ipd3N4 |
| Epitope | J038 binds to the V2-Apex. |
| Affinity | 7.6 nM |
| Isotype | Monkey IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; BLI: Neut; FC |
| Application Notes | ELISA: five-fold serially diluted antibody from 50 μg/mL was added into the wells with 1% BSA in PBS for 1 h incubation at 37°C. Bio-layer interferometry (BLI): 10 ng/mL monoclonal antibody was immobilized onto Anti-hIgG-Flow Cytometry Capture (AHC) biosensors. Neutralization assay (Neut): Purified antibody (50 μg/mL) was serially diluted at a 1:3 ratio with DMEM. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HIV-1 gp120 |
| Alternative Name | Human immunodeficiencyvirus-1 glycoprotein 120 |
| Research Area | Infectious Disease |
| Related Disease | Acquired Immune Deficiency Syndrome |
