| Description | This product is a mouse neutralizing antibody clone M4008_N1, which targets the gp120 of HIV-1. The clone was isolated using a VSV-displayed HIV-1 envelope trimer. The epitope of M4008_N1 is centered at the V3 crown. It can neutralize multiple variants that 45 of 120 tested strains. This antibody has been tested by Enzyme-linked immunosorbent assay and Neutralization assay. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | HIV-1 |
| Epitope | The epitope of M4008_N1 is centered at the V3 crown. |
| Neutralization Mechanism | M4008_N1 interacts with gp120 to form a β-sheet interaction with the V3 crown hairpin. M4008_N1 is sensitive to the surrounding glycans N197 and N301 but does not rely on them. |
| IC50 | ≤ 50 μg/mL |
| Isotype | Human IgG2 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Neut |
| Application Notes | ELISA: mAb was added at a starting concentration of 10 μg/mL in PBS with three-fold serial dilutions and incubated for 2 h. Neutralization assay (Neut): at a starting concentration of 10 μg/mL in PBS |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HIV-1 gp120 |
| Alternative Name | Human immunodeficiencyvirus-1 glycoprotein 120 |
| Research Area | Infectious Disease |
| Related Disease | Acquired Immune Deficiency Syndrome |
