| Description | This is a human antibody recognizing HIV-1 gp120, termed as M1214_N1. The epitope of M1214_N1 extends from V2 to V5 and centered on the ridge of the CD4-binding loop. It exhibited the best breadth (65%) and potency against the HIV-1. This product has been tested by Enzyme-linked immunosorbent assay, Flow cytometry and Neutralization assay. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | HIV-1 |
| Epitope | V2V5 corridor |
| Neutralization Breath | 65% (against HIV-1) |
| Isotype | Human IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; FC; Neut |
| Application Notes | ELISA: After blocking with 1% BSA in PBS at 37°C for 1 h, serially diluted antibody was incubated at 37°C for 1 h. Neutralization assay (Neut): 50 or 100 μL of antibody-virus mixture was incubated at 37°C for 30 min in duplicate wells before the addition of TZM-bl cells. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HIV-1 gp120 |
| Alternative Name | Human immunodeficiencyvirus-1 glycoprotein 120 |
| Research Area | Infectious Disease |
| Related Disease | Acquired Immune Deficiency Syndrome |
